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Are BEH300 C4 columns compatible with 100 percent aqueous mobile phase? - WKB193254

Article number: 193254

ENVIRONMENT

  • UltraPerformance Liquid Chromatography (UPLC)
  • Ultra High Performance Liquid Chromatography (UHPLC)
  • High Performance Liquid Chromatography (HPLC)
  • Bridged ethylene hybrid (BEH)
  • BEH300 C4 columns
  • ACQUITY
  • XBridge
  • water

ANSWER

Yes

ADDITIONAL INFORMATION

BEH300 C4 columns are compatible with 100% aqueous mobile phase, but there is a chance that dewetting will occur. It is recommended that you run in at least 5% organic solvent at all times if possible, to prevent the pores from dewetting (expelling this high aqueous solution). If dewetting occurs, you will see a decrease in retention times. To re-wet the packing, simply flush the column with organic solvent to re-wet the packed bed. If the aqueous mobile phase has a buffer, it is recommended that you first flush with 100% water with no buffer before flushing with organic, in order to avoid problems with salt precipitation.

Biomolecule Purification, Characterization, and Analyses Catalog

ACQUITY UPLC Protein BEH C4 300Å Columns Care and Use Manual

XBridge Protein BEH, C4, 300A, 3.5, 5 and 10 µm Columns Care and Use Manual

eluent, UPBINARY, UPSMPMGR

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