What are the recommended storage conditions for BEH SEC columns? - WKB192965

ENVIRONMENT

• UltraPerformance Liquid Chromatography (UPLC)
• Ultra High Performance Liquid Chromatography (UHPLC)
• High Performance Liquid Chromatography (HPLC)
• Size Exclusion Chromatography (SEC)
• Bridged ethylene hybrid (BEH)
• BEH SEC columns
• ACQUITY
• XBridge
• Protein separation
• Column storage

ANSWER

For overnight storage when the SEC column is on the LC system:
Continuously flush the column with the SEC mobile phase at 10-20% of the flow rate used for separations.

For short-term storage (<2 weeks), when the SEC column is removed from the LC system:
Tightly cap the column and store at 4 °C to prevent microbial growth. DO NOT FREEZE.

For long-term storage, when the SEC column is removed from the LC system:
Flush column (approx.10 column volumes) and store in a mobile phase containing 5–25 mM phosphate buffer, pH 6.5–7.0, and 20–100 mM (KCl or NaCl) with the addition of 10% acetonitrile, 20% methanol, or 0.05% sodium azide to prevent microbial growth. This storage solution can be a dilute version of the mobile phase with an antimicrobial added to it. To further mitigate the risk of microbial growth, tightly cap the column and store it at 4 °C. DO NOT FREEZE.

ADDITIONAL INFORMATION

Also see:

ACQUITY UPLC BEH SEC Columns  and Standards Care & Use Manual

XBridge Protein BEH SEC Columns & Standards Care & Use Manual