Can I use an Atlantis HILIC Silica column for a normal-phase separation after using it for a HILIC separation? - WKB190626

ENVIRONMENT

• High Performance Liquid Chromatography (HPLC)
• Hydrophilic interaction chromatography (HILIC)
• Polar compound retention
• United States Pharmacopeia (USP) L3 packing
• Solvent switching

ANSWER

Yes, but this is not recommended.

ADDITIONAL INFORMATION

The Atlantis HILIC silica packing is stable in 100% hexane. However, exposure to water, when running HILIC, will leave residual water adsorbed to the packing.  As a result, use of this column for normal-phase separation, after water exposure, may alter the selectivity or retention behavior of the packing.

Also see: Can the Atlantis HILIC Silica be switched into normal phase solvents?

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