How do I clean a µBondapak Cyano-RP column? - WKB125854

Article number: 125854

OBJECTIVE or GOAL

Clean a µBondapak CN-RP column.

Remove the guard cartridge.
Flush the column with 100% water for 10 column volumes.
Inject 200 µL of 50:50 dimethylsulfoxide/water every 5 minutes until 10 column volumes of water has passed through the column.
Stop injections.
Flush column with 10 column volumes of 100% water.
Flush column with 10 column volumes of 100% methanol.
Flush column with 10 column volumes of 100% chloroform.
Flush column with 10 column volumes of 100% methanol.
Flush column with 10 column volumes of 100% water.
Flush column with 10 column volumes of your initial mobile phase.

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