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Is it acceptable to reverse the direction of my ACQUITY BEH SEC column to clean it? - WKB121919

Article number: 121919

ENVIRONMENT

  • Column care and cleaning
  • ACQUITY BEH SEC

ANSWER

No. We do not recommend doing this for routine cleaning. 

ADDITIONAL INFORMATION

Back flushing the column should be considered as a last resort and done at a slow flow rate (i.e., 0.1 mL/min). However, this approach may further damage the column or only provide short-lived improvement in performance.

For additional information, see the ACQUITY UPLC Protein BEH SEC Columns and Standards Care and Use Manual.

id121919, UPBINARY

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