What is a good column choice for the analysis of vitamin D3? - WKB121786

ENVIRONMENT

• Vitamin D3
• Ultra Performance Liquid Chromatography (UPLC)
• Fat-soluble vitamins
• Oil-soluble vitamins
• Bridged ethylene hybrid (BEH)
• Charged surface hybrid (CSH)
• ACQUITY
• XBridge
• XSelect

ANSWER

For UPLC:
ACQUITY UPLC BEH C18
ACQUITY UPLC BEH Phenyl
ACQUITY UPLC CSH Phenyl-Hexyl

For UHPLC and/or HPLC:
XBridge BEH C18
XBridge BEH Phenyl
XSelect CSH Phenyl-hexyl

ADDITIONAL INFORMATION

The ACQUITY columns listed above were used in the following application notes.

The HPLC versions of these columns are listed in case a method is to be scaled to HPLC or UHPLC.

Quantification of 1,25-Dihydroxyvitamin D2 and D3 From Human Serum Using Immunopurification, ACQUITY UPLC, and Xevo TQ-S micro

Simultaneous Quantification of 25-Hydroxyvitamin D3, 25-Hydroxyvitamin D2, and 24,25-Dihydroxyvitamin D3 in Clinical Research Studies By UPLC-MS/MS

An LC-MS/MS Clinical Research Method for the Measurement of 25-OH Vitamin D2 and D3 Metabolites

Chromatographic Separation of Fat-Soluble Vitamins, Including the Two Vitamers D2 and D3