Is there a single SEC column that can be used with AAVs and mAbs? - WKB120192
Article number: 120192
ENVIRONMENT
- Size exclusion chromatography (SEC)
- SEC columns
- AAV
- mAb
ANSWER
No
ADDITIONAL INFORMATION
The pore size needed to accommodate separation of monomer from dimer of 20 nm sized AAV is too large for the 50-kd fragment of the mAbs. The 50-kd fragment falls outside the linear range of the SEC column.
For additional information, see the following:
Which SEC column is recommended for analysis of 20nm AAV?
What column is suggested for the SEC separation of mAb's and fAb's?
What is the molecular weight linear range for each of the BEH SEC columns?
id120192, monoclonal antibody