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What is the estimated back pressure the BioResolve SEC mAb 200 2.5 um 7.8 x 300 mm column generates on an LC system? - WKB120009

Article number: 120009

ENVIRONMENT

  • BioResolve SEC mAb
  • column
  • back pressure
  • LC
  • 7.8 x 300 mm

ANSWER

The estimated back pressure in an aqueous mobile phase with temperature 25 degrees Celsius at 0.6mL/min. is approximately 1600 psi (109 bar).

ADDITIONAL INFORMATION

The estimate is based on a standard HPLC system and tubing. This is a higher pressure on a UPLC system. 

For additional information, see What is the suggested column format for BioResolve SEC mAb column attached to ACQUITY systems? and BioResolve SEC mAb Guard and Columns Care and Use Manual.

id120009, eluent, monoclonal antibody

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