Can I expose my C18 column to a surfactant in my mobile phase? - WKB116854
Article number: 116854
ENVIRONMENT
- C18 column
- Mobile phase
ANSWER
Adding a surfactant such as polysorbate 80 or Tween 20 can substantially reduce the lifetime of the column. Surfactants on a column can form micelles (250A in diameter) in solutions and layers on surfaces.
ADDITIONAL INFORMATION
If a surfactant must be used in the mobile phase, dedicate the column to the method because the surface chemistry will be altered and consider using a guard column.
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