What is a good choice of nanoEase M/Z column for performing targeted lipidomics studies? - WKB116588

ENVIRONMENT

• Charged surface hybrid (CSH)
• Lipidomics
• nanoEase C18 column

ANSWER

CSH C18

ADDITIONAL INFORMATION

See the related application note, which uses a CHS C18 iKey separation device: Targeted Lipidomics Using the ionKey/MS System

nanoEase M/Z Columns Ordering Information

nanoEase M/Z Columns Care and Use Manual

NOTE:

For the following reasons, Waters does not recommend use of nanoscale LC for lipidomics applications:

1. Mass load onto an iKey separation device may be as much as 200 times lower than on an analytical-scale column such as a 2.1-mm ID column.
2. An iKey separation device may increase sensitivity up to 20 times vs. a 2.1-mm ID analytical column, but this may not overcome the drastic reduction in mass load. 

The dimensions of the iKey separation device used in the application note above were 150 µm ID x 100 mm length.