What buffer reagent is a good substitute for Sodium Perchlorate when used with a BEH SEC column? - WKB109623
Article number: 109623
ENVIRONMENT
- BEH SEC
- ACQUITY
- XBridge
- buffer
- reagent
- salt
- Size exclusion
- protein
ANSWER
This is a complex question because the choice of buffer in the mobile phase can have a significant effect on the SEC of proteins.
ADDITIONAL INFORMATION
The affect on proteins is more pronounced when you change from Perchlorate salt to Chloride salt.
For additional information, see What are some suggestions for doing method development for hydrophobic proteins using BEH SEC columns?
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