Can I use a BioResolve mAb Phenyl column for the analysis of protein aggregation (monomer from dimer)? - WKB108810
Article number: 108810
ENVIRONMENT
- BioResolve
- mAb
- Phenyl
- Column
- Aggregation
- Monomer
- Dimer
- Protein
- Column
- Bridged Ethylene Hybrid (BEH)
- Size Exclusion Chromatography (SEC)
ANSWER
No, because this column uses acetonitrile, which is a denaturing method.
ADDITIONAL INFORMATION
Instead, use BEH SEC columns for aggregate analysis:
Bioseparations and Analyses Catalog
Guide to Size-Exclusion Chromatography (SEC) of mAb Aggregates, Monomers, and Fragments
BEH SEC Column Technology – Infographic
id108810, monoclonal antibody