What is a good column choice for analysis of a quaternary amine compound? - WKB107141

ENVIRONMENT

• UltraPerformance Liquid Chromatography (UPLC)
• Ultra High Performance Liquid Chromatography (UHPLC)
• High Performance Liquid Chromatography (HPLC)
• ACQUITY columns
• Column
• XSelect columns
• Charged surface hybrid (CSH)
• Hydrophilic interaction chromatography (HILIC)
• Bridged ethylene hybrid (BEH)

ANSWER

Reverse phase:
ACQUITY UPLC CSH C18 at low pH (if compound is not extremely polar)
XSelect CSH C18 at low pH (if compound is not extremely polar)
Atlantis PREMIER BEH C18 AX at high pH (higher than pH 8.5)

HILIC:
CORTECS HILIC
ACQUITY UPLC BEH HILIC
ACQUITY UPLC BEH Amide
XBridge BEH HILIC
XBridge BEH Amide
 

ADDITIONAL INFORMATION

If the analyte is very polar, HILIC may be the preferred separation technique.

In reverse phase, use of ion pairing may help.