What is the sequence of the pepsin in the Enzymate Pepsin Column (186007233) used in the HDX experiments? - WKB97911

ENVIRONMENT

• SYNAPT G2-S
• SYNAPT G2-Si
• SYNAPT XS
• Xevo G2-S
• Xevo G2-XS
• HDX

ANSWER

As part of the Waters HDX solution, digestion is carried out online by immobilized pepsin on Enzymate Pepsin Column (186007233). For data processing/analysis, pepsin sequence should be included in the protein sequences database.

MKWLLLLSLVVLSECLVKVPLVRKKSLRQNLIKNGKLKDFLKTHKHNPASKYFPEAAALIGDEPLENYLDTEYFGTIGIGTPAQDFTVIFDTGSSNLWVPSVYCSSLACSDHNQFNPDDSSTFEATSQELSITYGTGSMTGILGYDTVQVGGISDTNQIFGLSETEPGSFLYYAPFDGILGLAYPSISASGATPVFDNLWDQGLVSQDLFSVYLSSNDDSGSVVLLGGIDSSYYTGSLNWVPVSVEGYWQITLDSITMDGETIACSGGCQAIVDTGTSLLTGPTSAIANIQSDIGASENSDGEMVISCSSIDSLPDIVFTINGVQYPLSPSAYILQDDDSCTSGFEGMDVPTSSGELWILGDVFIRQYYTVFDRANNKVGLAPVA

ADDITIONAL INFORMATION

Enzymate BEH Pepsin Columns Care and Use Manual

Pepsin contains three disulphide bridges and a phosphorylated serine. More information in the Swissprot entry - P00791