What are the amino acid sequences of the Waters standards 186008927, 186009125, and 186009126? - WKB96794

ENVIRONMENT

• The following three Waters standards are produced from the same mAb protein. The NIST mAb (humanized IgG1_k) monoclonal antibody is a recombinant antibody expressed in murine suspension culture. 

  • Humanized mAb Mass Check Standard [186009125]

    • The sample consists of the intact mAb made of two light chains (LC) and two heavy chains (HC) linked with 16 disulfide bridges. The average molar mass should be 145179.8052 g/mol without modifications.

  • mAb Subunit Standard [186008927]

    • The sample consists of the Humanized mAb Mass Check Standard [186009125], which is reduced and digested with Ides. The Ides enzyme cleaves the heavy chain between the glycine-glycine amino acid residues. This sample consist of three sequences: Fc/2, Fd', and LC.

  • mAb Tryptic Digest Standard [186009126]

    • This sample consist of the Humanized mAb Mass Check Standard [186009125], which is reduced and alkylated to cleave the disulfide bridges. Then it is digested with trypsin. This sample consists of the two sequences HC and LC.

ANSWER

mAb Subunit Fc/2 sequence (Average molar mass: 23790.6425)

GPSVFLFPPK  PKDTLMISRT  PEVTCVVVDV  SHEDPEVKFN  WYVDGVEVHN  AKTKPREEQY  NSTYRVVSVL  TVLHQDWLNG
KEYKCKVSNK  ALPAPIEKTI  SKAKGQPREP  QVYTLPPSRE  EMTKNQVSLT  CLVKGFYPSD  IAVEWESNGQ  PENNYKTTPP
VLDSDGSFFL  YSKLTVDKSR  WQQGNVFSCS  VMHEALHNHY  TQKSLSLSPGK

mAb Subunit Fd' sequence (Average molar mass: 25705.8931)

QVTLRESGPA  LVKPTQTLTL  TCTFSGFSLS  TAGMSVGWIR  QPPGKALEWL  ADIWWDDKKH  YNPSLKDRLT  ISKDTSKNQV  VLKVTNMDPA
DTATYYCARD  MIFNFYFDVW  GQGTTVTVSS  ASTKGPSVFP  LAPSSKSTSG  GTAALGCLVK  DYFPEPVTVS  WNSGALTSGV  HTFPAVLQSS
GLYSLSSVVT  VPSSSLGTQT  YICNVNHKPS  NTKVDKRVEP  KSCDKTHTCP  PCPAPELLG

mAb LC (Average molar mass: 23127.5093)

DIQMTQSPST  LSASVGDRVT  ITCSASSRVG  YMHWYQQKPG  KAPKLLIYDT  SKLASGVPSR  FSGSGSGTEF  TLTISSLQPD  DFATYYCFQG
SGYPFTFGGG  TKVEIKRTVA  APSVFIFPPS  DEQLKSGTAS  VVCLLNNFYP  REAKVQWKVD  NALQSGNSQE  SVTEQDSKDS  TYSLSSTLTL
SKADYEKHKV  YACEVTHQGL  SSPVTKSFNR  GEC

mAb HC (Average molar mass: 49478.5203)

QVTLRESGPA  LVKPTQTLTL  TCTFSGFSLS  TAGMSVGWIR  QPPGKALEWL  ADIWWDDKKH  YNPSLKDRLT  ISKDTSKNQV  VLKVTNMDPA
DTATYYCARD  MIFNFYFDVW  GQGTTVTVSS  ASTKGPSVFP  LAPSSKSTSG  GTAALGCLVK  DYFPEPVTVS  WNSGALTSGV  HTFPAVLQSS
GLYSLSSVVT  VPSSSLGTQT  YICNVNHKPS  NTKVDKRVEP  KSCDKTHTCP  PCPAPELLGG  PSVFLFPPKP  KDTLMISRTP  EVTCVVVDVS
HEDPEVKFNW  YVDGVEVHNA  KTKPREEQYN  STYRVVSVLT  VLHQDWLNGK  EYKCKVSNKA  LPAPIEKTIS  KAKGQPREPQ  VYTLPPSREE
MTKNQVSLTC  LVKGFYPSDI  AVEWESNGQP  ENNYKTTPPV  LDSDGSFFLY  SKLTVDKSRW  QQGNVFSCSV  MHEALHNHYT  QKSLSLSPGK

ADDITIONAL INFORMATION