What is the sequence of the antibody (186006552) used in the HDX SST? - WKB96707

ENVIRONMENT

• SYNAPT G2-S/Si
• SYNAPT XS
• HDX

ANSWER

As part of the system suitability for HDX experiments, customers planning to analyze proteins with disulfide bridges might want to test their system with a monoclonal antibody standard (186006552 - Intact mAb Mass Check Standard) reconstituted in equilibration buffer to a concentration of 12.0 µM for a Synapt G2-Si system, 40.0 µM for a Synapt G2 system.

Light Chain Sequence - C₁₀₆₆H₁₆₄₉N₂₈₇O₃₄₃S₇ - Avg. MW = 24197.7 Da

DVLMTQTPLSLPVSLGDQASISCRSSQYIVHSNGNTYLEWYLQKPGQSPKLLIYKVSNRFSGVPDRFSGSGSGTDFTLKISRVEAEDLGVYYCFQGSHVPLTFGAGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC

Heavy Chain Sequence - C₂₁₇₀H₃₃₃₈N₅₆₂O₆₆₁S₁₉ - Avg. MW = 48484.3 Da

QVQLKESGPGLVAPSQSLSITCTVSGFSLLGYGVNWVRQPPGQGLEWLMGIWGDGSTDYNSALKSRISITKDNSKSQVFLKMNSLQTDDTAKYYCTRAPYGKQYFAYWGQGTLVTVSAAKTTPPSVYPLAPGSAAQTDSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSETVTCNVAHPASSTKVDKKIVPRDCGCKPCICTVPEVSSVFIFPPKPKDVLTITLTPKVTCVVVDISKDDPEVQFSWFVDDVEVHTAHTQPREEQFNSTFRSVSELPIMHQDWLNGKEFKCRVNSAAFPAPIEKTISKTKGRPKAPQVYTIPPPKEQMAKDKVSLTCMITDFFPEDITVEWQWNGQPAENYKNTQPIMDTDGSYFVYSKLNVQKSNWEAGNTFTCSVLHEGLHNHHTEKSLSHSPG

Intact protein - C₆₄₇₂H₉₉₄₀N₁₆₉₈O₂₀₀₈S₅₂ - Avg. MW = 145,329.7 Da

The glutamine at the N-terminus of both heavy chains forms Pyroglutamic Acid.

The protein contains 17 disulfide bridges (* 1: and 4: light chains, 2: and 3: heavy chains).

1:23 - 1:93          2:222 - 3:222     3:22 - 3:95          4:23 - 4:93

1:139 - 1:199     2:225 - 3:225     3:145 - 3:200     4:139 - 4:199

1:219 - 2:220     2:227 - 3:227     3:220 - 4:219    

2:22 - 2:95          2:256 - 2:316     3:362 - 3:420    

2:145 - 2:200     2:362 - 2:420     3:256 - 3:316    

ADDITIONAL INFORMATION

Intact mAb Mass Check Standard Care and Use Manual