Does Waters have a recommended protein hydrolysis method for AccQ•Tag? - WKB77492

ENVIRONMENT

• Amino acid analysis (AAA)
• AccQ•Tag
• AccQ•Tag Ultra
• Hydrolysis

ANSWER

No

ADDITIONAL INFORMATION

There are many techniques for performing protein hydrolysis.

See United States Pharmacopeia (USP) General Chapter <1052> BIOTECHNOLOGY-DERIVED ARTICLES—AMINO ACID ANALYSIS for details on protein hydrolysis techniques.

Also see the Waters Comprehensive Guide to Hydrolysis and Analysis of Amino Acids.

and journal articles:

Michael Fountoulakis and Hans-Werner Lahm, Hydrolysis and Amino Acid Composition of proteins; J. Chromatography A 1998 Nov 27;826(2);109-34.

doi: 10.1016/s0021-9673(98)00721-3.

M. Weiss, M. Manneberg, J F. Juranville, H W. Lahm, M. Fountoulakis, Effect of the hydrolysis method on the determination of the amino acid composition of proteins;

J. Chromatography A 1998 Feb 6;795(2):263-75. doi: 10.1016/s0021-9673(97)00983-7.