What is the gradient table that was used to generate data for application note 720006482EN (Application of Difluoroacetic Acid to Improve Optical and MS Performance in Peptide LC-UV/MS)?

ENVIRONMENT

• Application notes
• IonHance
• Difluoroaceitic acid (DFA)
• Gradient table

ANSWER

The Quaternary Solvent Manager (QSM) gradient used to generate the MassPREP Peptide data with 0.1% DFA:

Time (min)   Flow Rate (mL/min)    %A    %B    Curve
Initial            0.500                           95        5     Initial
2.0               0.500                           95         5     6
37.0             0.500                           60       40     6
40.5             0.500                           35       65     6
45.5             0.500                           35       65     6 
49.5             0.500                           95         5     6
60.0             0.500                           95         5     6

ADDITIONAL INFORMATION

Application of Difluoroacetic Acid to Improve Optical and MS Performance in Peptide LC-UV/MS?

MassPREP Peptide Mixture Care and Use Manual

Ionhance Difluoracetic Acid Infographic

IonHance Difluoroacetic Acid Care and Use Manual

Enhancing Subunit-Level Profiling of mAbs and ADCs with MSQuality Difluoroacetic Acid