What is the concentration of the protein (prior to digestion) in each vial of the MassPREP Digestion Standard Mix 1 (186002865) and the MassPREP Digestion Standard Mix 2 (186002866)? - WKB68307
ENVIRONMENT
- Analytical standards and reagents
- MassPREP Protein Digestion Standards
ANSWER
These standards are considered qualitative standards and are not meant to be used for quantitative purposes.
The following values are nominal values, not exact concentrations in a given lot.*
186002865 - MassPREP Digestion Standard Mix 1 contains:
(molar ratio, amount)*
Yeast Alcohol Dehydrogenase 1.0, 50 pmol
Phosphorylase B 1.0, 50 pmol
Yeast Enolase 1.0, 50 pmol
Bovine Serum Albumin 1.0, 50 pmol
186002866 - MassPREP Digestion Standard Mix 2 contains:
(molar ratio, amount)*
Yeast Alcohol Dehydrogenase 1.0, 50 pmol
Phosphorylase B 0.5, 25 pmol
Yeast Enolase 2.0, 100 pmol
Bovine Serum Albumin 8.0, 400 pmol
*Refer to the Certificate of Analysis (CoA) for lot-specific information; there is
additional quality control (QC) testing done for accuracy per vial of each protein
digest.
ADDITIONAL INFORMATION
See MassPREP Digestion Standard Mixtures Care & Use Manual
Waters does sell a quantitative peptide standard:
186006555 - Quantitative Peptide Retention Standard
Also see:
Quantitative Peptide Retention Standard Care & Use Manual
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