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What is the concentration of the protein (prior to digestion) in each vial of the MassPREP Digestion Standard Mix 1 (186002865) and the MassPREP Digestion Standard Mix 2 (186002866)? - WKB68307

Article number: 68307

ENVIRONMENT

  • Analytical standards and reagents
  • MassPREP Protein Digestion Standards

ANSWER

These standards are considered qualitative standards and are not meant to be used for quantitative purposes.

The following values are nominal values, not exact concentrations in a given lot.*

186002865 - MassPREP Digestion Standard Mix 1 contains:
                                                   (molar ratio, amount)*
Yeast Alcohol Dehydrogenase        1.0, 50 pmol
Phosphorylase B                             1.0, 50 pmol
Yeast Enolase                                 1.0, 50 pmol
Bovine Serum Albumin                   1.0, 50 pmol

186002866 - MassPREP Digestion Standard Mix 2 contains:
                                                    (molar ratio, amount)*
Yeast Alcohol Dehydrogenase         1.0, 50 pmol
Phosphorylase B                              0.5, 25 pmol
Yeast Enolase                                  2.0, 100 pmol
Bovine Serum Albumin                    8.0, 400 pmol

*Refer to the Certificate of Analysis (CoA) for lot-specific information; there is 
additional quality control (QC) testing done for accuracy per vial of each protein
digest.

ADDITIONAL INFORMATION

See MassPREP Digestion Standard Mixtures Care & Use Manual

Waters does sell a quantitative peptide standard:

186006555 - Quantitative Peptide Retention Standard

Also see:

Quantitative Peptide Retention Standard Care & Use Manual

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