When preparing mobile phase, should the pH be adjusted before or after adding the organic? - WKB61993
Article number: 61993
ENVIRONMENT
- Mobile phase preparation
ANSWER
Adjust the pH before adding the organic portion of the mobile phase.
ADDITIONAL INFORMATION
If the method was developed by adjusting the pH after adding the organic, the method may work better by doing the same. pH meters are not calibrated in organic and are not intended to be used in this way; readings will not be accurate.
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