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When preparing mobile phase, should the pH be adjusted before or after adding the organic? - WKB61993

Article number: 61993

ENVIRONMENT

  • Mobile phase preparation

ANSWER

Adjust the pH before adding the organic portion of the mobile phase.

ADDITIONAL INFORMATION

If the method was developed by adjusting the pH after adding the organic, the method may work better by doing the same. pH meters are not calibrated in organic and are not intended to be used in this way; readings will not be accurate. 

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