How do I prepare AccQ•Tag Ultra eluent B for my ACQUITY UPLC H-Class? - WKB55134

ENVIRONMENT

• AccQ•Tag Ultra
• Amino acid analysis
• ACQUITY UPLC H-Class

ANSWER

To prepare the 90:10 water/AccQ•Tag Ultra eluent B mobile phase for ACQUITY UPLC H-Class (and H-Class Bio) systems:

1. Measure 900 mL of water into a 1-L graduated cylinder and transfer the
water to an appropriately labeled mobile phase reservoir.
2. In another graduated cylinder, measure 100 mL of AccQ•Tag Ultra
eluent B (186003839).
3. Add the AccQ•Tag Ultra eluent B to the mobile phase reservoir
containing the water, and mix thoroughly for at least 5 minutes.

ADDITIONAL INFORMATION

Tip: The AccQ•Tag Ultra eluent B and water solution remains
serviceable for 3 days when stored at room temperature.