If I follow the standard RapiZyme protocol with guanidine HCl, do I still need to add the Tris Calcium Chloride buffer salt? - WKB257604
Article number: 257604
ENVIRONMENT
- RapiZyme Trypsin
- Guanidine HCl
- Tris Calcium Chloride
ANSWER
If the Tris Calcium Chloride buffer salt is not present, RapiZyme Trypsin will still digest the protein.
ADDITIONAL INFORMATION
Use of the Tris Calcium Chloride enhances the activity of RapiZyme Trypsin and, in buffered conditions at pH 7.5, reduces some non-specific cleavage.
For additional information about using guanidine HCl only, see the one-pot (no desalt) sample preparation in the following application note:
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