What are the masses of the oligonucleotides in the MassPREP Oligonucleotide Standard (186004135)? - WKB253323

ENVIRONMENT

• Oligonucleotides
• Oligonucleotide Separations Technology (OST)
• MassPREP Oligonucleotide Standard

ANSWER

Average mass values can differ based on the type of mass spectrometer used, as well as the software used to calculate these values.
  
The Certificate of Analysis (C of A) for this standard provides the following average masses:

• 35T = 10584.930
• 30T =   9063.945
• 25T =   7542.960
• 20T =   6021.975
• 15T =   4500.990

ADDITIONAL INFORMATION

Here is an example of average masses as calculated on a BioAccord System with an RDa mass detector: 

• 35T = 10584.7967 
• 30T =   9063.8308
• 25T =   7542.8649 
• 20T =   6021.8990 
• 15T =   4500.9331 

Also see:
An Automated Compliance-Ready LC-MS Workflow for Intact Mass Confirmation and Purity Analysis of Oligonucleotides
MassPREP OST Standard Care & Use Manual