How to make sodium formate for QTof calibrations - WKB2458

OBJECTIVE or GOAL

How to make sodium formate for QTof calibrations

ENVIRONMENT

• SYNAPT G2 MS/HDMS
• SYNAPT G2-S MS/HDMS
• SYNAPT G2-Si MS/HDMS
• SYNAPT G2-S
• SYNAPT G2-Si
• Vion IMS QTof
• Xevo G2 QTof
• Xevo G2 Tof
• Xevo G2-S QTof
• Xevo G2-S Tof
• Xevo G2-XS QTof
• Xevo G2-XS Tof
• MassLynx 4.1
• UNIFI

PROCEDURE

5 mM sodium formate solution in 90:10 2-propanol/water
1. Using a 1000-μL pipette, transfer 1000 μL of 0.1 M sodium hydroxide solution to a 20-mL volumetric flask.
2. Using a 1000-μL pipette, transfer 900 μL of deionized or LCMS-grade water to the 20-mL volumetric flask.
3. Using a 200-μL pipette, add 100 μL formic acid (186006922-5) to the 20-mL volumetric flask.
4. Make up to 20 mL with 90:10 2-propanol/water and sonicate for 5 minutes.
5. Label as "5 mM sodium formate solution in 90:10 2-propanol/water" and store in the refrigerator (expires in 1 week).
6. For Fluidics systems, transfer from the 20-mL volumetric flask to a 30-mL fluidics container and label as "5 mM sodium formate solution in 90:10 2-propanol/water" and store in the refrigerator (expires in 1 week).

0.5 mM sodium formate solution in 90:10 2-propanol/water
1. Using a 1000-μL pipette, add 2000 μL of 5 mM sodium formate solution to a 20-mL volumetric flask.
2. Make up to 20 mL with 90:10 2-propanol/water and sonicate for 5 minutes.
3. Label as "0.5 mM sodium formate solution in 90:10 2-propanol/water" and store in the refrigerator (expires in 1 week).
4. For Fluidics systems, transfer from the 20-mL volumetric flask to a 30-mL fluidics container and label as "0.5 mM sodium formate solution in 90:10 2-propanol/water" and store in the refrigerator (expires in 1 week).

ADDITIONAL INFORMATION

The 0.1 M sodium hydroxide solution can be found in several Waters kits click this link to see what kits are available. Anything with a green box with a blue i next to it is a chemical kit. 

Alternatively the 0.1M sodium hydroxide solution can be made by dissolving 0.4g of sodium hydroxide in 100 mL of water.