What standard is recommended for finding the void peak (Vo) for the Styragel GPC columns in DMF mobile phase? - WKB239964
Article number: 239964
ENVIRONMENT
- Styragel columns
- Void peak
- Excluded peak
- DMF
- GPC
ANSWER
Polystyrene standards run in DMF with 50mM LiBr mobile phase additive; polymethylmethacrylate standards run in DMF mobile phase.
ADDITIONAL INFORMATION
The standard should be a high-molecular-weight standard that is outside the linear range of the column bank.
For additional information, see the Beginner's Guide to Size-Exclusion Chromatography.
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