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How much protein should I use with my RapiGest SF digestion protocol? - WKB206011

Article number: 206011

ENVIRONMENT

  • RapiGest SF

ANSWER

The quantity of starting protein can vary depending on the levels of detection needed and the type of protein to be digested.

ADDITIONAL INFORMATION

Optimization of the digestion parameters must be done, and this can vary by the particular protein and digestion enzyme.

For additional information, see Improving UPLC-FLR-MS N-glycan Analysis of Disulife-rich Fusion Proteins through Optimization of Sample Preparation

and Rapigest SF Surfactant: An Enabling Tool for In-Solution Enzymatic Protein Digestions

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