Can I reconstitute 1 mg of RapiGest SF in 100 µL of a 10X cell lysis buffer? - WKB194435
Article number: 194435
ENVIRONMENT
- RapiGest
- Solubilization Factor (SF)
- Acid labile surfactant
- Protein
- Denature
- Reconstitute
ANSWER
Yes
ADDITIONAL INFORMATION
Waters sees no issues with this proposal.
For example, a 10X cell lysis buffer might contain all of the following constituents:
- 20 mM Tris HCL
- 150 mM sodium chloride
- 1 mM sodium EDTA
- 1 mM EGTA
- 2.5 mM sodium pyrophosphate
- 1 mM B-glycrophosphate
- 1 mM sodium orthovanadate
RapiGest SF Surfactant Care & Use Manual
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