What is the amino acid sequence for the Waters Monoclonal Antibody (mAb) Check Standard (186006552)? - WKB12299

ENVIRONMENT

• LCMS
• Standards and reagents
• mAb mass check
• Amino acid sequence

ANSWER

The Waters Intact Monoclonal Antibody Check Standard is an LCMS standard used as a qualitative tool for intact mass analysis and designed for routine MS optimization, system setup, and performance testing. 

 

Protein Information

The mAb consists of two light chains (light blue) and two heavy chains (dark blue): 

Intact Protein Formula C₆₄₇₂ H₉₉₄₀ N₁₆₉₈ O₂₀₀₈ S₅₂

Average MW = 145,329.7

• 17 disulfide bonds 
• N-terminus of each heavy chain has a fixed pyroglutamic acid from Q
• One N-linked glycosylation on each heavy chain. Total Number of Glycoforms: 4 (G0, G0F, G1F, G2F)
• Disclaimer: I/L are exchangeable

 

The sequence for mAb is as follows: 

Light Chain

DVLMTQTPLSLPVSLGDQASISCRSSQYIVHSNGNTYLEWYLQ

KPGQSPKLLIYKVSNRFSGVPDRFSGSGSGTDFTLKISRVEAED

LGVYYCFQGSHVPLTFGAGTKLEIKRADAAPTVSIFPPSSEQLTS

GGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSK

DSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC

 

Heavy Chain

QVQLKESGPGLVAPSQSLSITCTVSGFSLLGYGVNWVR

QPPGQGLEWLGMIWGDGSTDYNSALKSRITISKDNSKS

QVFLKMNSLQTDDTAKYYCTRAPYGKQYFAYWGQGTLV

TVSAAKTTPPSVYPLAPGSAAQTDSMVTLGCLVKGYFPEP

VTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTW

PSETVTCNVAHPASSTKVDKKIVPRDCGCKPCICTVPEVS

SVFIFPPKPKDVLTITLTPKVTCVVVDISKDDPEVQFSWFV

DDVEVHTAHTQPREEQFNSTFRSVSELPIMHQDWLNGKE

FKCRVNSAAFPAPIEKTISKTKGRPKAPQVYTIPPPKEQMA

KDKVSLTCMITDFFPEDITVEWQWNGQPAENYKNTQPIM

DTDGSYFVYSKLNVQKSNWEAGNTFTCSVLHEGLHNHH

TEKSLSHSPG

 

ADDITIONAL INFORMATION

For additional information, see the Intact mAb Mass Check Standard and the Intact mAb Mass Check Standard Care and Use Manual.