Will adding NaOH to the borate buffer used to derivatize amino acids with AccQ•Tag change the retention time of peaks in the chromatography? - WKB120887
Article number: 120887
ENVIRONMENT
- Amino acid analysis
- AccQ•Tag
- AccQ-Fluor
- NaOH
- neutralization
ANSWER
No
ADDITIONAL INFORMATION
The addition of NaOH to the borate buffer can be a way of ensuring the derivatization takes place in the pH range best suited for efficient derivatization of the primary and secondary amines. It is typically done if the sample is suspected of harboring very acidic salts, which would bring the pH of the sample in the borate buffer below the optimal pH range for fast and selective derivatization of the amines.
For additional information see Derivatization guidelines in Comprehensive Guide to Hydrolysis and Analysis of amino acids
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