What can cause inadequate or incomplete derivatization of amino acids by AccQ-Tag reagent? - WKB117475
Article number: 117475
ENVIRONMENT
- AAA
- AccQ-Tag
- cause
- stressed reaction
- incomplete
- inadequate
- tag
- label
- derivatization
ANSWER
The conditions that can cause inadequate derivatization include:
1) Too much sample.
2) The pH of the reaction is not ideal.
3) The reagent is too old or not properly stored upon reconstitution.
4) Not promptly mixing the reaction/sample after adding the derivatization reagent.
ADDITIONAL INFORMATION
For additional information, see How long can reconstituted AccQ-Tag reagent be stored?
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