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What is the source of the NH3 peak in the AccQ•Tag derivatization reaction? - WKB117459

Article number: 117459

ENVIRONMENT

  • AccQ•Tag derivatization
  • Amino acid analysis (AAA)

ANSWER

The NH3 peak comes from the ammonia present in the water environment and air, the breakdown of amino acids occurring in the sample, and any other source of ammonia or nitrogen that could be present in the lab.

ADDITIONAL INFORMATION

The NH3 peak is always present in the derivatization reaction but it varies in area because of the changing environment and atmosphere. Note that it is present, but do not use it as an internal standard.

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