What is the optimized cleaning procedure for trifluoroacetic acid (TFA)? - WKB109900

ENVIRONMENT

• Cleaning protocols
• Trifluoroacetic acid (TFA)

ANSWER

Optimized cleaning protocol for TFA

Prime solution: 5% FA in 25:25:25:25 water/MeCN/MeOH/IPA

Wash solution: 2% FA in 25:25:25:25 water/MeCN/MeOH/IPA

1. Remove sinkers from lines and switch to 5% prime solution using an “air-gap” + “inbetween” solvent changing technique (Original MP > air gap > inbetween vessel > air gap > wash > wash solution).

    Be sure to wipe away any residual TFA solution before submerging into the 5% prime solution.

2. Prime all lines in contact with TFA for 60 minutes with 5% prime solution.

3. After priming, switch to 2% wash solution using an “air gap” + “inbetween” solvent changing technique; be sure to wipe away any residual 5% prime solution before submerging into the 2% wash solution.

4. Run 2% wash solution at 0.5 mL/min for a minimum of 2 hours to waste. Do not run high %FA through MS detector.

5. Run series of intact mAb on Bioresolve RP column to check for signal intensity and adduct levels. Check for continuously increasing signal intensity.

ADDITIONAL INFORMATION