Which hydroxylysine is the D and L form when tagged and separated by AccQ•Tag Ultra cell culture method conditions? - WKB108188
Article number: 108188
ENVIRONMENT
- AccQ•Tag Ultra
- Cell culture
- Amino acid analysis
- Hydroxylysine
ANSWER
The Hydroxylysine standard commonly used for the cell culture separation contains both L and D forms. In nature, the L form is the norm.
ADDITIONAL INFORMATION
Typically, L and D forms are not separated under reverse-phase conditions.
However, in this method, L and D isomers of hydroxylysine are separated.
- m/z ratios show the presence of both mono-derivatized and di-derivatized forms within the two peaks.